Lab 5 Mitosporic fungi survey

Objective:
Observe conidia morphology of different fungi.
Method:
Regular mounting technique
Results:
1. Botrytis cinera: conidia are borne in grape-like clusters. Conidia are hyaline, ellipsoid to obovoid. There is contamination in Botrytis cinera. There are two conidia forms  present in the view. Based on my study, the small round ones are Botrytis cinera conidia.

2. Curvularia sp.: The poroconidia are curved slightly to distinctly, transversely septate, with an expanded third cell from the pore end of the conidium.

 

3. Epicoccum: Conidia look round, non-septate, brown to black in color, with both transverse and oblique septa, which makes them resemble a soccer ball.

4. Monilinia Fructicola: Conidia are hyaline (colorless), lemon-shaped, and produced in a moniloid manner (resembling a string of beads with constricted ends).

5. Rhizoctonia Solani: it does not produce spores and is hence identified only from mycelial characteristics or DNA analysis. Its hyphal cells are multinucleated. The hyphae are 4–15 μm wide and tend to branch at right angles. A septum near each hyphal branch and a slight constriction at the branch.

6. Thielaviopsis Basicola:  two types of conidia:

Type 1 conidial chains: terminal, with dark grey walls, smooth, in chains of (2)3-9 so appearing multiseptate, shortly cylindric with flat ends.

Type 2 phialoconidia: hyaline, smooth, cylindric, flat ended, very variable in size with 3-7 septate towards base.